Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity

Authors

  • João Tiago Correia Oliveira Rural Federal University of Pernambuco (Brasil)
  • Gilka Talita Silva Rural Federal University of Pernambuco (Brasil)
  • Williane Patrícia da Silva Diniz Rural Federal University of Pernambuco (Brasil)
  • Everthon Fernandes Figueredo Rural Federal University of Pernambuco (Brasil)
  • Isaneli Batista dos Santos University of São Paulo (Brasil)
  • Danubia Ramos Moreira de Lima Rural Federal University of Pernambuco (Brasil)
  • Maria Carolina Quecine University of São Paulo (Brasil)
  • Júlia Kuklinsky- Sobral Rural Federal University of Pernambuco (Brasil)
  • Fernando José Freire Rural Federal University of Pernambuco (Brasil)

DOI:

https://doi.org/10.7764/rcia.v45i3.1949

Keywords:

N-Acyl homoserine lactones, plant growth promoting bacteria, plant-microbe interactions

Abstract

J. T. C. Oliveira, G. T. Silva, W. P. S. Diniz, E. F. Fernandes, I. B. Santos, D. R. M. Lima, M.C.

Quecine, J. Kuklinsky-Sobral, and F. J. Freire. 2018. Diazotrophic bacteria isolated from

Brachiaria spp.: genetic and physiological diversity. Cien. Inv. Agr. 45(3): 277-289. Grass from

the genus Brachiaria spp. predominates in pastures with low fertile soils. This scenario highlights

the importance of the association with microorganisms to foster plant growth, which becomes

essential to the successful establishment of this forage in such environments. This study aimed to

evaluate the genetic variability and identify the mechanisms of plant growth promotion, in vitro,

of bacteria associated with Brachiaria decumbens Stapf. and Brachiaria humidicola (Rendle.)

Schweickerdt in Pernambuco, Brazil. We evaluated 20 isolates of diazotrophic bacteria obtained

from the endophyte or rhizosphere communities. The genetic characteristics were determined

via sequencing the 16S rRNA region, which allowed us to identify ten different bacterial genera:

Bacillus sp., Burkholderia sp., Enterobacter sp., Klebsiella sp., Microbacterium sp., Pantoea sp.,

Ralstonia sp., Rhizobium sp., Sinomonas sp., and Sphingomonas sp., with a specificity of the genus

Rhizobium sp. to Brachiaria decumbens Stapf.. The phenotypic and functional characteristics

revealed that 100% of the bacterial strains produced indol-3-acetic acid (IAA) with the addition

of L-tryptophan, and 60% presented IAA production independent of the L-tryptophan pathway.

We also detected that 70% of the isolated bacteria possessed the capacity to solubilize phosphorus.

The analysis of the enzymatic output revealed that 30% of the bacterial isolates produced cellulase,

60% produced pectate lyase, 15% produced polygalacturonase, and 30% produced amylase.

We also detected the production of N-acyl homoserine lactones in 65% of bacterial strains. In

summary, our results showed that plants of B. decumbens Stapf. and B. humidicola (Rendle.)

Schweickerdt interacted with different bacterial genera capable of promoting plant growth.

Author Biographies

João Tiago Correia Oliveira, Rural Federal University of Pernambuco (Brasil)

Rural Federal University of Pernambuco, Academic Unit of Garanhuns. Avenida Bom Pastor, s/n - Boa

Vista, Garanhuns, Pernambuco, Brazil.

Gilka Talita Silva, Rural Federal University of Pernambuco (Brasil)

Rural Federal University of Pernambuco, Academic Unit of Garanhuns. Avenida Bom Pastor, s/n - Boa

Vista, Garanhuns, Pernambuco, Brazil.

Williane Patrícia da Silva Diniz, Rural Federal University of Pernambuco (Brasil)

Rural Federal University of Pernambuco, Academic Unit of Garanhuns. Avenida Bom Pastor, s/n - Boa

Vista, Garanhuns, Pernambuco, Brazil.

Everthon Fernandes Figueredo, Rural Federal University of Pernambuco (Brasil)

Rural Federal University of Pernambuco, Academic Unit of Garanhuns. Avenida Bom Pastor, s/n - Boa

Vista, Garanhuns, Pernambuco, Brazil.

Isaneli Batista dos Santos, University of São Paulo (Brasil)

University of São Paulo, Superior School of Agriculture “Luiz de Queiroz”, Department of Genetics.

Avenida Pádua Dias, 11 - Agronomia, Piracicaba, São Paulo, Brazil.

Danubia Ramos Moreira de Lima, Rural Federal University of Pernambuco (Brasil)

Rural Federal University of Pernambuco, Department of Agronomy. Av. Dom Manoel de Medeiros, s/n,

Recife, Pernambuco, Brazil.

Maria Carolina Quecine, University of São Paulo (Brasil)

University of São Paulo, Superior School of Agriculture “Luiz de Queiroz”, Department of Genetics.

Avenida Pádua Dias, 11 - Agronomia, Piracicaba, São Paulo, Brazil.

Júlia Kuklinsky- Sobral, Rural Federal University of Pernambuco (Brasil)

Rural Federal University of Pernambuco, Academic Unit of Garanhuns. Avenida Bom Pastor, s/n - Boa

Vista, Garanhuns, Pernambuco, Brazil.

Fernando José Freire, Rural Federal University of Pernambuco (Brasil)

Rural Federal University of Pernambuco, Department of Agronomy. Av. Dom Manoel de Medeiros, s/n,

Recife, Pernambuco, Brazil.

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Published

2018-12-26

Issue

Vol. 45 No. 3 (2018)

Section

RESEARCH ARTICLE

License

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This work is licensed under a Creative Commons Attribution 4.0 International License.