Symptoms of viral infection have been observed during the last decade in berry crops; however, only few viral diseases have been reported on raspberry (Rubus idaeus), red currant (Ribes sanguineum) and blueberry (Vaccinium corymbosum) crops in Chile. A serological survey based on ELISA test was performed in2000 to determine the six most important virus affecting raspberries and red currants and the four most important viruses in blueberries. This survey comprised a total of 82 ha representative of the main cultivated area distributed in about 940 km from north to south. The results obtained demonstrated an extremely high incidence of the following viruses in raspberry: Apple mosaic virus (87-98%), Arabis mosaic virus (29-86%), Raspberry bushy dwarf virus (35-68%) and Tomato ringspot virus (63-85%). Low incidence levels of Tomato ringspot virus (0-14%) and Tobacco ringspot virus (6-19%) were found in blueberries. Red currants had a low incidence of either Arabis mosaic virus or Tomato ringspot virus, in just one location each. Therefore, the results obtained in this survey will be useful for the establishment of a virus-free certification program, based on ELISA test in Chile. To our knowledge, with the exception of Tomato ringspot virus and Raspberry bushy dwarf virus in raspberry, these virus were reported for the first time, in the above mentioned host plants, in Chile.