In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile

Matilde E.  Uribe; Kelly  Paredes; Catherine  Delaveau; Pedro  Carrasco; Carlos  Baeza; Freddy  Mora

RESEARCH ARTICLE

Vol. 41 No. 2 (2014)

In vitro bulbing for the propagation of Traubia modesta (Amaryllidaceae), a threatened plant endemic to Chile

Matilde E. Uribe⁺⁻
Kelly Paredes⁺⁻
Catherine Delaveau⁺⁻
Pedro Carrasco⁺⁻
Carlos Baeza⁺⁻
Freddy Mora⁺⁻

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Submitted: July 2, 2021
Published: 2014-08-29

Abstract

Critically endangered, Traubia modesta is endemic to Chile and belongs to the family Amaryllidaceae. In this research, a propagation protocol was developed for the in vitro cultivation of vegetative organs for this species. The twin scale explants were cultured in a Murashige and Skoog (MS) medium, supplemented with the growth regulators naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) in different concentrations. Plant preservative mixture (PPM) and polyvinyl pyrrolidone (PVP) were used as a biocide and an antioxidant, respectively. The results showed high contamination of the bulbs. There were no significant differences between the treatments with plant growth regulators. A multiplication rate of 1.3 – 2.2 for bulbils was achieved, with an average of 28 bulbils per mother bulb. The natural regeneration rate is approximately 1 - 4 bulbils per mother bulb once a year.

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