Root-knot nematodes, particularly Meloidogyne ethiopica, represent a major constraint to crop production in Chile. While plant growth-promoting rhizobacteria and entomopathogenic nematode symbionts are known sources of nematicidal metabolites, their combined activity against plant-parasitic nematodes (PPNs) has received little attention. In this study, the nematicidal activity of crude extracts obtained from plant growth-promoting rhizobacteria (Bacillus mycoides FB25M and Peribacillus frigoritolerans FB37BR) and symbiotic bacteria associated with entomopathogenic nematodes (Xenorhabdus bovienii LR, Photorhabdus thracensis UCH-913, and Photorhabdus antumapuensis UCH-873) was evaluated. Extracts were tested individually and in mixtures against second-stage juveniles (J2) and eggs of M. ethiopica in vitro, followed by a preliminary greenhouse assay. In vitro assays revealed that crude extracts significantly increased J2 mortality at both tested concentrations (25% and 50%), with high mortality after 72 h (p<0.001). No recovery occurred after transfer to water, indicating a nematicidal effect. Compared with that of the control, egg hatching strongly decreased during early incubation, with a reduction of up to 95%. However, the observed inhibitory effects diminished over time, likely because of the progressive depletion of eggs in the control. In contrast, the crude extracts did not significantly reduce the nematode infection parameters under greenhouse conditions, and these differences were attributable only to the chemical control. These results highlight the nematicidal potential of metabolites from rhizobacteria and entomopathogenic nematode symbionts against M. ethiopica. Further research is required to understand the stability and persistence of these bacterial metabolites in soil and to develop formulation strategies that enhance their efficacy.